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jetprime buffer  (Sartorius AG)


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    Structured Review

    Sartorius AG jetprime buffer
    Jetprime Buffer, supplied by Sartorius AG, used in various techniques. Bioz Stars score: 99/100, based on 11273 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/jetprime+buffer/jetPRIME+DNA%2FsiRNA/pmc13040135-52-20-25
    Average 99 stars, based on 11273 article reviews
    jetprime buffer - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Method for producing t cells modified by chimeric antigen receptor
    Article Snippet: .. Specific steps are as follows. (1) The 293FT cell culture flask (T175) that had reached 80%-90% was taken out from the cell incubator in 5% CO2 at 37° C., the cells were collected and washed after digestion, 4.5×106 cells and 9 mL of DMEM complete medium (purchased from Gibco, product catalog No.: 11965-084) were added to each 10 cm-cell culture dish and gently shaken, and the resulting mixture was put in the incubator in 5% CO2 at 37° C. (2) On the second day of culturing, the following reagents were added to each culture dish: 500 μL of jetPRIME® buffer (purchased from Polyplus Transfection Co., product catalog No.: B161116), 6 μg of recombinant lentivirus plasmid, 3 μg of psPAX2 (purchased from Wuhan Miaoling Biotechnology Co., Ltd., product catalog No.: P026) and 1.5 μg of pMD2.G (purchased from Guangzhou Geneseed Biotechnology Co., Ltd., product catalog No.: 161220L08) and then mixed well, and then jetPRIME® (purchased from Polyplus Transfection Co., product catalog No.: 114-15) was added to the system at 25 μL per 10 cm-culture dish and mixed well, and the resulting mixture was left to stand at room temperature for 10 min to obtain a mixed solution. (3) The 293FT cells used for packaging viruses were taken out of the cell incubator in 5% CO2 at 37° C., the mixed solution was added to each culture dish and the shaken gently, and the resulting mixture was put in the incubator in 5% CO2 at 37° C. for continuous culturing. ..

    Recombinant:

    Article Title: Method for producing t cells modified by chimeric antigen receptor
    Article Snippet: .. Specific steps are as follows. (1) The 293FT cell culture flask (T175) that had reached 80%-90% was taken out from the cell incubator in 5% CO2 at 37° C., the cells were collected and washed after digestion, 4.5×106 cells and 9 mL of DMEM complete medium (purchased from Gibco, product catalog No.: 11965-084) were added to each 10 cm-cell culture dish and gently shaken, and the resulting mixture was put in the incubator in 5% CO2 at 37° C. (2) On the second day of culturing, the following reagents were added to each culture dish: 500 μL of jetPRIME® buffer (purchased from Polyplus Transfection Co., product catalog No.: B161116), 6 μg of recombinant lentivirus plasmid, 3 μg of psPAX2 (purchased from Wuhan Miaoling Biotechnology Co., Ltd., product catalog No.: P026) and 1.5 μg of pMD2.G (purchased from Guangzhou Geneseed Biotechnology Co., Ltd., product catalog No.: 161220L08) and then mixed well, and then jetPRIME® (purchased from Polyplus Transfection Co., product catalog No.: 114-15) was added to the system at 25 μL per 10 cm-culture dish and mixed well, and the resulting mixture was left to stand at room temperature for 10 min to obtain a mixed solution. (3) The 293FT cells used for packaging viruses were taken out of the cell incubator in 5% CO2 at 37° C., the mixed solution was added to each culture dish and the shaken gently, and the resulting mixture was put in the incubator in 5% CO2 at 37° C. for continuous culturing. ..

    Plasmid Preparation:

    Article Title: Method for producing t cells modified by chimeric antigen receptor
    Article Snippet: .. Specific steps are as follows. (1) The 293FT cell culture flask (T175) that had reached 80%-90% was taken out from the cell incubator in 5% CO2 at 37° C., the cells were collected and washed after digestion, 4.5×106 cells and 9 mL of DMEM complete medium (purchased from Gibco, product catalog No.: 11965-084) were added to each 10 cm-cell culture dish and gently shaken, and the resulting mixture was put in the incubator in 5% CO2 at 37° C. (2) On the second day of culturing, the following reagents were added to each culture dish: 500 μL of jetPRIME® buffer (purchased from Polyplus Transfection Co., product catalog No.: B161116), 6 μg of recombinant lentivirus plasmid, 3 μg of psPAX2 (purchased from Wuhan Miaoling Biotechnology Co., Ltd., product catalog No.: P026) and 1.5 μg of pMD2.G (purchased from Guangzhou Geneseed Biotechnology Co., Ltd., product catalog No.: 161220L08) and then mixed well, and then jetPRIME® (purchased from Polyplus Transfection Co., product catalog No.: 114-15) was added to the system at 25 μL per 10 cm-culture dish and mixed well, and the resulting mixture was left to stand at room temperature for 10 min to obtain a mixed solution. (3) The 293FT cells used for packaging viruses were taken out of the cell incubator in 5% CO2 at 37° C., the mixed solution was added to each culture dish and the shaken gently, and the resulting mixture was put in the incubator in 5% CO2 at 37° C. for continuous culturing. ..

    Article Title: A cascade via CD276/PI3K/SIRT1/E-Cad in overcoming contact inhibition of proliferation in hepatocellular carcinoma cells
    Article Snippet: These molecular cloning procedures were conducted by Guangzhou RiboBio Co., Ltd. On Day 0, a total of 2x10 6 cells were seeded into a 35 mm dish with 2 ml of serum-supplemented DMEM. .. Proceeding to Day 1, once cell confluence reached ~50%, 2 μg of plasmid DNA were diluted in 200 μl of jetPRIME buffer (cat. no. 101000046, POLYPLUS-TRANSFECTION S.A; https://shop.sartorius.com.cn/cn/p/jetprime-dnasirna-co-transfection/jetPRIME_DNA_siRNA_co_transfection ), followed by vortex and brief centrifugation. ..

    Article Title: Inducible, split base editors for in vivo cancer functional genomics.
    Article Snippet: .. Transfections were performed using 50 μl of jetPRIME buffer, 2.4 μl of jetPRIME reagent (Polyplus) and 0.6 μg of plasmid DNA. ..

    other:

    Article Title: A brain-gut excitatory peptide/CCHamide homolog regulates satiation and motivational state transitions in the Aplysia feeding circuit.
    Article Snippet: IP3 is generated by Gαq pathway when a GPCR expressed in CHO-K1 cells (Procell, CL0062) is activated by an appropriate ligand.

    Article Title: DNA damage burden causes selective CUX2 neuron loss in neuroinflammation.
    Article Snippet: On day 4, 96-well plates were coated with poly-l-lysine (0.1 mg ml−1, Sigma-Aldrich, P1524) for 1 h at 37 °C.

    Injection:

    Article Title: Distinct miR319a identified from Persicaria chinensis mediates cross-kingdom suppression of cervical cancer by targeting ITGA3.
    Article Snippet: One week after tumor cell implantation, each mouse was intratumorally injected with 5 μg of pch-miR319a plasmid or control vector, which was mixed in 50 μL of Opti-MEMTM (Gibco) containing Lipofectamine 3000 transfection reagent. .. To investigate the role of pch- Yan et al. Acta Biochim Biophys Sin xxxx miR319a, pch-miR319a mimics or a scrambled miRNA (5 μM) mixed with 5 μL of jetPRIME reagent and 100 μL jetPRIME buffer (Polyplus, Illkirch, France) were administered by subcutaneous injection to each mouse. ..

    Transfection:

    Article Title: A brain-gut excitatory peptide/CCHamide homolog regulates satiation and motivational state transitions in the Aplysia feeding circuit
    Article Snippet: .. In preliminary experiments, for each dish (6-well plate), 2 μg of the putative receptor plasmids in pcDNA3.1(+) (1.5 μg plasmids, when co-transfecting with 1.5 μg Gα16) were mixed with 200 μl jetPRIME buffer (Polyplus, 101000046) in a 1.5 mL EP tube, followed by the addition of 4 μl (6 μl, when co-transfecting with Gα16) of jetPRIME Transfection reagent (Polyplus, 101000046) mixture. ..

    Article Title: Inhibition of HDAC3 Expression Alleviates High–Glucose‐Induced Photoreceptor Cell Apoptosis and Oxidative Stress
    Article Snippet: .. For each transfection, 4 μ L of siRNA was diluted in 200 μ L of jetPRIME buffer, mixed with 4 μ L of jetPRIME transfection reagent (Polyplus‐transfection S.A., Illkirch, France), and incubated according to the manufacturer′s instructions. ..

    Article Title: Inducible, split base editors for in vivo cancer functional genomics.
    Article Snippet: .. Transfections were performed using 50 μl of jetPRIME buffer, 2.4 μl of jetPRIME reagent (Polyplus) and 0.6 μg of plasmid DNA. ..

    Centrifugation:

    Article Title: A cascade via CD276/PI3K/SIRT1/E-Cad in overcoming contact inhibition of proliferation in hepatocellular carcinoma cells
    Article Snippet: These molecular cloning procedures were conducted by Guangzhou RiboBio Co., Ltd. On Day 0, a total of 2x10 6 cells were seeded into a 35 mm dish with 2 ml of serum-supplemented DMEM. .. Proceeding to Day 1, once cell confluence reached ~50%, 2 μg of plasmid DNA were diluted in 200 μl of jetPRIME buffer (cat. no. 101000046, POLYPLUS-TRANSFECTION S.A; https://shop.sartorius.com.cn/cn/p/jetprime-dnasirna-co-transfection/jetPRIME_DNA_siRNA_co_transfection ), followed by vortex and brief centrifugation. ..

    Incubation:

    Article Title: Inhibition of HDAC3 Expression Alleviates High–Glucose‐Induced Photoreceptor Cell Apoptosis and Oxidative Stress
    Article Snippet: .. For each transfection, 4 μ L of siRNA was diluted in 200 μ L of jetPRIME buffer, mixed with 4 μ L of jetPRIME transfection reagent (Polyplus‐transfection S.A., Illkirch, France), and incubated according to the manufacturer′s instructions. ..



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